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However, if NAC was given four hours after the overdose, there was no reduction in liver toxicity, and 80% of the mice died

Note: Results from glutathione supplementation vary from person to person

Interestingly, the variants of surface exposed residues belong to two different domains of FXN within the NFS1-ISCU-FXN complex: the region spanning Glu96 to Asp124, corresponding to the first -helix of FXN that is involved in the interaction with NFS1, and the region spanning Asn146 to Arg165 composing the -sheet that is involved in the interaction with ISCU (Figure 4D)

The mechanisms are multi-layered: inotropic effects via calcium influx, anti-fibrotic actions through PPARgamma upregulation, anti-inflammatory effects via NFkB suppression, and cell survival through PI3K/AKT1 pathway activation (Berlanga-Acosta et al., 2017)